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27 Feb 2008 Our software library is known for having the same amount of. 1 year ago Jan. Scaricare Bidoo Hack Torrent 5610The effect of opioids on cytoskeletal protein phosphorylation in PC12 cells.
The purpose of the present studies was to investigate the effect of opioids on the phosphorylation of cytoskeletal proteins in PC12 pheochromocytoma cells. For these studies, PC12 cells were preincubated with the opioid agonist DAMGO, followed by cell stimulation with forskolin. Cells were exposed to tyrosine kinase and tyrosine phosphatase inhibitors, in order to elucidate the effect of opioid actions on tyrosine kinase activity. As observed, DAMGO increases protein kinase C (PKC) activity, although this effect was prevented by the PKC inhibitor, staurosporine. Furthermore, DAMGO did not block staurosporine-induced cytoskeletal protein phosphorylation suggesting that, although DAMGO increases PKC activity, its effects on cytoskeletal protein phosphorylation are independent of PKC. In contrast, the inhibitory effect of DAMGO on interleukin-1-induced cytoskeletal protein phosphorylation was reversed by the PKC inhibitor, which suggests an inhibitory effect of opioids on interleukin-1-induced tyrosine kinase activity. Taken together, these data suggest that opioid treatment of PC12 cells increases PKC activity, but inhibits interleukin-1-induced tyrosine kinase activity./*
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